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Turicensis C. muytjensii C. dublinensis Genomospecies group 1 Totala177 (99) 173 (98) 0 (0) 172 (97) 87 (49) 90 (51) 47 (27) 0 (0) 35 (19) 137 (77) 25 (one hundred) 0 (0) 18 (72) 0 (0) 0 (0) 0 (0) 0 (
Kado and Lui (35) plasmid preparations from these five strains and from the plasmid-harboring and plasmidcured derivatives of C. sakazakii strain BAA-894 had been subjected to agarose gel electrophoresis to confirm the repA PCR final results (see Fig. S3 inside the supplemental material). Except for C. muytjensii E488, four from the 5 repA PCR-negative strains, as well as the pESA3-cured derivative of C.Turicensis C. muytjensii C. dublinensis Genomospecies group 1 Totala177 (99) 173 (98) 0 (0) 172 (97) 87 (49) 90 (51) 47 (27) 0 (0) 35 (19) 137 (77) 25 (100) 0 (0) 18 (72) 0 (0) 0 (0) 0 (0) 0 (0) 21 (84) 25 (100) 0 (0) 6 (one hundred) 0 (0) six (one hundred) 0 (0) 1 (16) 0 (0) 0 (0) three (50) six (one hundred) 0 (0) 9 (75) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) five (83) 0 (0) 1 (20) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) two (100) two (one hundred) 0 (0) 0 (0) 0 (0) PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25486018 0 (0) 0 (0) two (one hundred) two (one hundred) 0 (0)0 (0) 177 (100) 177 (one hundred) 0 (0) 25 (100) 25 (100) 0 (0) six (one hundred) 6 (one hundred) 9 (one hundred) 9 (one hundred) 1 (11) 5 (one hundred) five (one hundred) 1 (20) 0 (0) two (100) 2 (one hundred)224 (97)175 (78)25 (11)172 (76) 87 (38) 87 (38) 47 (20) 26 (11)69 (30)137 (61) 14 (6)224 (100) 212 (95)14 (6)Numbers inside parentheses will be the percent PCR constructive for every single gene locus in relation for the total number of plasmid-harboring strains of that species, except for the SAR405838 information for repA, which are in relation to the total number of strains.pCTU1 and conservation of pESA2 in BAA-894 and pCTU2 and pCTU3 in z3032 had been confirmed by plasmid extraction and agarose gel electrophoresis (curing of pESA3 is shown in Fig. S3 in the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26344672 supplemental material). In addition, the plasmidcured derivatives of C. sakazakii BAA-894 and C. turicensis z3032 have been PCR adverse for all pESA3/pCTU1 plasmid targets (data not shown). Furthermore, BAA-894 and z3032 wildtype and plasmid-cured derivative strains had been PCR positive for the repA gene of pESA2/pCTU2, whereas PCR assays created to detect the pCTU3 repA gene were optimistic in the wild-type strain and plasmid-cured derivative of C. turicensis z3032 (data not shown). Taken with each other, these benefits demonstrated that the plasmid-cured derivatives of those strains lacked only pESA3 or pCTU1 plasmids, respectively. Detection of RepFIB plasmids among Cronobacter spp. based on repA-targeted PCR. A total of 229 strains of Cronobacter spp. were screened by PCR, using primers targeting the repA gene, for the presence of a pESA3- or pCTU1-like plasmid. Among the six species groups, 224 strains (97 ) have been PCR constructive for repA (Table two). The five repA PCR-negative strains incorporated 3 C. muytjensii strains, E456, E488, and ATCC 51329, C.
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