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Wang J, Zhang H, Wang H. Evaluation of predicted CD8+ T
2008; three:e2375. [PubMed: 18584054] Zvi A, Ariel N, Fulkerson J, Sadoff JC, Shafferman A. Entire genome identification of Mycobacterium tuberculosis vaccine candidates by comprehensive data mining and bioinformatic analyses. BMC Med Genomics. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21795619 2008; 1:18. [PubMed: 18505592]Author Manuscript Author Manuscript Author Manuscript Author ManuscriptPathog Dis. Author manuscript; readily available in PMC 2015 April 01.Kerns et al.PageAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptPathog Dis. Author manuscript; out there in PMC 2015 April 01.Figure 1.(A) 5 week-old Mycobacterium tuberculosis pellicle PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27010563 grown statically in Sauton‘s Minimal Media as described in Ojha et al 2008 and (B) a Fluorescence In-Situ Hybridization stain with the pellicle for MTB 16s rRNA (100?magnification).Kerns et al.PageAuthor Manuscript Author Manuscript Author ManuscriptFigure two.Author ManuscriptTwo-dimensional gel electrophoresis (��)-VeraliprideMedChemExpress (��)-Veralipride protein-stained gels of (A) 2 week shaken culture, (C) three week pellicle, (E) five week pellicle, and (G) 7 week pellicle membrane proteins (Sypro Ruby Stain). Two-dimensional Western Blots of (B) two week shaken culture, (D), 3 week pellicle, (F) 5 week pellicle, and (H) 7 week pellicle membrane proteins probed with 60-day MTB infected Guinea Pig serum and visualized with horseradish-peroxidase labeled secondary antibody.Pathog Dis. Author manuscript; readily available in PMC 2015 April 01.Kerns et al.PageAuthor Manuscript Author ManuscriptFigure three.Pellicle Fexinidazole custom synthesis Specific protein conservation determined by tBLASTn of protein sequences against translated genomes. M. tuberculosis bar represents median conservation with the protein amongst 15 sequenced MTB strains.Author Manuscript Author ManuscriptPathog Dis. Author manuscript; available in PMC 2015 April 01.Table IComprehensive list of antigenic proteins identified grouped by expression patterns (Pellicle Specific, Universal, or Indeterminate)Kerns et al.Protein expression was established for every timepoint by either mass-spectrometry (MS) or Sypro-Ruby (SR) protein stain of two-dimensional gels. Massspectrometry identities had been determined with an expectation worth threshold of 10-3 or less, Sypro-Ruby spot identities were representative off a minimum of three replicate 2D gels for every timepoint/sample. Protein more protein info like isoelectric point, molecular weight, and functional information and facts obtained from Tuberculist (tuberculist.epfl.ch).Rv# pI MW (kDa) 2W Shaken 3.Wang J, Zhang H, Wang H. Analysis of predicted CD8+ T cell epitopes from proteins encoded by the precise RD regions of Mycobacterium tuberculosis for vaccine improvement and distinct diagnosis. Mol Biol Rep. 2010; 37:1793?799. [PubMed: 19609720] Weichart D, Querfurth N, Dreger M, Hengge-Aronis R. Global part for ClpP-containing proteases in stationary-phase adaptation of Escherichia coli. J Bacteriol. 2003; 185:115?25. [PubMed: 12486047] WHO. International tuberculosis report 2012. Planet Heath Organization; 2012. Zhang L, Wang Q, Wang W, et al. Identification of putative biomarkers for the serodiagnosis of drugresistant Mycobacterium tuberculosis. Proteome Sci. 2012; 10:12. [PubMed: 22364187] Zhang Y, Heym B, Allen B, Young D, Cole S. The catalase-peroxidase gene and isoniazid resistance of Mycobacterium tuberculosis. Nature. 1992; 358:591?93. [PubMed: 1501713] Zheng H, Lu L, Wang B, et al. Genetic basis of virulence attenuation revealed by comparative genomic analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv. PLoS 1.
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