点评详情
发布于:2018-6-11 08:14:41  访问:78 次 回复:0 篇
版主管理 | 推荐 | 删除 | 删除并扣分
Ure 2 Diminished transmission of M184 variants by DCs. DCs had been exposed
Cells were stained with antibodies Stattic structure against CD1a as marker for DCs and intracellular p24 for HIV infection and analyzed by flow cytometry. Retrovirology 2014, 11:113 http://www.retrovirology.com/content/11/1/Page 4 ofFigure 3 The diminished transmission of M184 variants will not be caused by replication in target cells. A: To identify the replication capacity of the virus panel in target cells, CCR5+ Jurkat T cells have been infected inside the absence of drugs and p24 production was monitored daily. Average infection with typical deviation is depicted. B-C: LCs (B) or DCs (C) have been exposed to the equivalent of 17.five (open bars) or 100 ng (closed bars) p24 for 4 days, extensively washed and co-cultured for two days with TZM-bl cells pre-incubated with PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28154141 indinavir. Infection was measured by luminescence in comparison to HIV-WT. get AZD3759 Information are representative for the typical with SD for 1 (LCs) and 2 (DCs) donors in duplo. Abbreviations: WT: HIV-WT, M184V: HIV-M184V, M184I: HIV-M184I, M184T: HIV-M184T, K103N: HIV-K103N, n.c.: no infection control.variants have been less frequently transmitted as HIV-WT plus the HIV-K103N transmission efficacy was comparable to HIV-WT (Figure 3B-C). In agreement with all the LC transmission experiments, the infection degree of LCs by HIV-K03N was intermediate (Figure 5).Ure two Diminished transmission of M184 variants by DCs. DCs were exposed for the equivalent of 17.5 (open bars) or 100 ng (closed bars) p24 for 4 days, extensively washed and co-cultured for two days with CCR5+ Jurkat T cells. Cells have been stained with antibodies against CD1a as marker for DCs and intracellular p24 for HIV infection and analyzed by flow cytometry. A: The percentage of infected target cells (CD1a adverse cells) at 2 days post transmission (dpt) (6 days post infection; dpi) (n = 4, representative for 3/4 donors). B: The percentage of infected target cells (CD1a adverse cells) at 1, 2 and 4 days post transmission (n = two, 100 ng p24 infection is shown). Abbreviation: n.c.: no infection control.Pingen et al. Retrovirology 2014, 11:113 http://www.retrovirology.com/content/11/1/Page four ofFigure three The diminished transmission of M184 variants is just not triggered by replication in target cells. A: To establish the replication capacity with the virus panel in target cells, CCR5+ Jurkat T cells had been infected in the absence of drugs and p24 production was monitored everyday. Average infection with regular deviation is depicted. B-C: LCs (B) or DCs (C) were exposed towards the equivalent of 17.five (open bars) or one hundred ng (closed bars) p24 for 4 days, extensively washed and co-cultured for two days with TZM-bl cells pre-incubated with PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28154141 indinavir. Infection was measured by luminescence compared to HIV-WT. Data are representative for the average with SD for 1 (LCs) and two (DCs) donors in duplo. Abbreviations: WT: HIV-WT, M184V: HIV-M184V, M184I: HIV-M184I, M184T: HIV-M184T, K103N: HIV-K103N, n.c.: no infection manage.variants had been significantly less often transmitted as HIV-WT along with the HIV-K103N transmission efficacy was comparable to HIV-WT (Figure 3B-C).
共0篇回复 每页10篇 页次:1/1
共0篇回复 每页10篇 页次:1/1
我要回复
回复内容
验 证 码
看不清?更换一张
匿名发表 
当前位置
脚注信息

美博梁sir2010-2017版权所有